Coding

Part:BBa_K105101:Design

Designed by: Katja Karstens   Group: iGEM08_ESBS-Strasbourg   (2008-08-12)


Gal4 - DNA binding domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 134


Design Notes

To enable the fusion with other domains in frame the vector of this BioBricks has no base pair in between the restriction side and the BioBrick (Phillips, I.E. and Silver, P.A. "A new Biobrick Assembly Strategy Designed for Facile Protein Engineering." DSpace http://hdl.handle.net/1721.1/32535 (2006).



Source

plasmid from the local lab containing a Gal4-VP16 chimera

References